Coding

Part:BBa_K4940016:Design

Designed by: Kuang Minjie   Group: iGEM23_iZJU-China   (2023-10-11)


Prα-POL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 71
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the gene has a clear sequence and has been proven useful based on many experiments, no certain considerations were in the design process.


Source

This gene has been sequenced from Pseudomonas rhodesiae  CIP107491.

References